293t  (ATCC)

Journal: Biochemistry and Biophysics Reports

Article Title: Ectopic endometrial mesenchymal stem cell-derived exosomal miR-4466 promotes angiogenesis by targeting RUNX1 in adenomyosis

doi: 10.1016/j.bbrep.2026.102457

Figure Lengend Snippet: miR-4466 directly regulates RUNX1 expression in HUVECs. (A) A Venn diagram shows target genes of miR-4466 predicted by the miRDB, miRWalk, miRPathDB, mirDIP databases. (B) Schematic diagram illustrating the predicted miR-4466 binding sites with the 3′ UTR of RUNX1. (C, D) Relative expression of target genes was detected by qRT-PCR in HUVECs (C) and HEK-293T cells (D) transfected with NC-mimics and miR-mimics. n = 3. E, F Relative expression of target genes was detected by qRT-PCR in HUVECs (E) and HEK-293T cells (F) transfected with NC-inhibitor and miR-inhibitor. n = 3. (G) Schematic representation of the wild-type (Wt) and mutant-type (Mut) binding site between the 3′ UTR of RUNX1 and miR-4466. (H) Relative luciferase activity of reporter constructs containing miRNA binding sites transfected with NC-mimics and miR-mimics in HUVECs. n = 3. (I) Western blot of RUNX1 and VEGFA protein expression in HUVECs after transfection with NC-mimics/inhibitor or miR-mimics/inhibitor for 48 h (Full-length blot are presented in Supplementary Figure). (J, K) Protein levels of RUNX1 (J) and VEGFA (K) were normalized to GAPDH protein expression level in (I). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001 and ns: P > 0.05.

Article Snippet: Human umbilical vein endothelial cells (HUVECs) and human embryonic kidney 293T (HEK-293T) cells were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA).

Techniques: Expressing, Binding Assay, Quantitative RT-PCR, Transfection, Mutagenesis, Luciferase, Activity Assay, Construct, Western Blot

Journal: Alzheimer's & Dementia

Article Title: NRN1 as a therapeutic target for Alzheimer's disease

doi: 10.1002/alz.71149

Figure Lengend Snippet: Abcam ab64186 recognizes NRN1 protein in mouse neuroblastoma cells. (A) Representative Western blot of human embryonic kidney (HEK) 293T, Neuro‐2a (N2a) mouse neuroblastoma, and SH‐SY5Y human neuroblastoma cell lysates (50 µg protein), probed with NRN1 polyclonal antibody Abcam ab64186 and GAPDH monoclonal antibody. (B) N2a cells were transfected with NRN1 or Scramble (non‐targeting) siRNA smart pools and harvested after 96 h. Western blot analyses with NRN1 polyclonal antibody Abcam ab64186 revealed reduced intensity of the ∼34 kDa band in NRN1‐depleted cells, compared to Scramble control. 10 µg of protein was loaded per lane, and GAPDH was probed as a loading control. GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; NRN1, Neuritin‐1; siRNA, small interfering RNA;

Article Snippet: Human embryonic kidney (HEK) 293T cell lines (Catalog No.: CRL‐3216, ATCC) were used.

Techniques: Western Blot, Transfection, Control, Small Interfering RNA